Study programmes / C-HE Horticultural Engineering / Plant tissue cultures in horticulture
Course code:EKZ
Course title in language of instruction:Explantátové kultury v zahradnictví
Course title in Czech:Explantátové kultury v zahradnictví
Course title in English:Plant tissue cultures in horticulture
Mode of completion and number of credits:Exam (4 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes:full-time, 1/2
(full-time, hours of lectures per week / hours of seminars per week)
Language of instruction:Czech
Level of course:master
Semester:SS 2012/2013 - FH
Name of lecturer:doc. Mgr. Miroslav Baránek, Ph.D. (supervisor)
Prerequisites:(Plant Physiology and (Botany I or Botany II)) or (Bachelor State Exam)
Aims of the course:Studying of in vitro cultivation techniques is the objective of this subject. The subject is focused on history and present in teh Czech Republic and in Europe. Student will study principles and uses of in vitro techniquies in hortitulture, e.g. virus elimination, genetic resources long-term maintenance, kryopreservation.

The subject study material was prepared with the support of OPVK project "Inovace studijních programů zahradnických oborů, reg. Č. CZ.1.07/2.2.00/28.0220".
Course contents:
1.Plant tissue cultures in horticulture (allowance 14/28)
a.Introduction and history
b.Laboratory organisation
c.Sterilization and collection of plant material
e.Plant growth regulators
g.Somatic embryogenesis
h.Rooting in vitro
i.Culture of plant protoplasts and somatic hybridisation
k.Production of disease-free plants
l.Using in practice, Czech and Slovak laboratories
m.Genetic characteristic of plant tissue cultures
n.Differences in anatomy and morphology of in vitro and in vivo plants
o.Production of secondary metabolites
p.Production of haploid plants in vitro, in vitro fertilization and embryo cultivation, zygotic embryo culture

Learning outcomes and competences:
Generic competences:
-ability to apply knowledge
-ability to create new ideas (creativity)
-ability to organize and plan ahead
-ability to work independently
-general knowledge
-science and research skills

Specific competences:
-Experimental work skills
-Knowledge necessary for management of in vitro laboratory
-Knowledge of ornamental plants reproduction
-Management of research and commercial laboratory
-Understanding of in vitro plant multiplication technology

Type of course unit:optional
Year of study:Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement:There is no compulsory work placement in the course unit.
Recommended study modules:none
Assessment methods:80% presence on practical courses, establish and evaluation of the individual in vitro experiment. Prepraring of 3 protocols about experiments. Oral exam.
Learning activities and study load (hours of study load)
Type of teaching methodDaily attendance
Direct teaching
     lecture14 h
     practice28 h
     professional educational excursion8 h
     preparation for exam30 h
     preparation for regular assessment10 h
     preparation of presentation12 h
     elaboration of reports10 h
Total112 h

Basic reading list
  • ROBERTS, L W. Experiments in Plant Tissue Culture. 3rd ed. CAmbridge: Cambridge University Press, 1995. 256 p. ISBN 0-521-47892-8.
  • RAZDAN, M K. Introduction to plant tissue culture. 2nd ed. Enfield: Science Publishers, 2003. 375 p. ISBN 1-57808-237-4.
  • GAMBORG, O L. -- PHILLIPS, G C. Plant cell, tissue and organ culture : fundamental methods. Berlin: Springer Verlag, 1995. 358 p. ISBN 3-540-58068-9.
  • GEORGE, E F. Plant propagation by tissue culture :. 3rd ed. Dordrecht: Springer, 2007. 501 p.
  • KŘIŽAN, B. et al. Metodika ozdravování česneku od virů pomocí kultivace meristému a in vitro kultur: [certifikovaná metodika]. Praha: Výzkumný ústav rostlinné výroby, 2010. 24 p. ISBN 978-80-7375-491-4.
  • GEORGE, E F. -- PUTTOCK, D J M. Plant culture media : Vol.1: Formulations and Uses. Edington: Exegetics Ltd., 1987. 6 p. ISBN 0-9509325-2-3.