Study programmes / C-HE Horticultural Engineering / Applied Plant Biotechnology
Course code:ARBT
Course title in language of instruction:Aplikované rostlinné biotechnologie
Course title in Czech:Aplikované rostlinné biotechnologie
Course title in English:Applied Plant Biotechnology
Mode of completion and number of credits:Exam (6 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes:full-time, 2/4
(full-time, hours of lectures per week / hours of seminars per week)
Language of instruction:Czech
Level of course:master; master continuing
Semester:SS 2014/2015 - FH
Name of lecturer:doc. Mgr. Miroslav Baránek, Ph.D. (instructor, lecturer, supervisor)
Ing. Kateřina Baránková, Ph.D. (instructor, lecturer)
Ing. Aleš Eichmeier, Ph.D. (instructor, lecturer)
Ing. Martina Kudělková, Ph.D. (instructor, lecturer)
Mgr. Jana Raddová, Ph.D. (instructor, lecturer)
Prerequisites:Bachelor State Exam
Aims of the course:Main goal of this subject is to acquaint students with practical applications of plant biotechnology methods and their use in plant growing practice. On the base of conveniently selected laboratory exercises students will handle experiments based on up to date principles (restriction enzymes, PCR, Real Time PCR, reverse transcription, in vitro multipliciation, in vitro tehrmotherapy ...).
Course contents:
1.Classical breeding methods (allowance 2/2)
2.The main trends in the utilization of biotechnology in horticulture, the relationship of biotechnology and classical breeding (allowance 2/2)
3.Methods of molecular genetics not manipulating the genetic information of studied organism (allowance 12/32)
a.Isolation of DNA from plant tissues
b.Polymerase chain reaction ( PCR) and its preparation
c.The molecular genetic methods based on fragmentation analysis
d.Use of DNA markers for assessment of genetic relatedness
e.Use of SSR markers in identifying varieties
f.Use of Real Time PCR for quantification of GMO material in an unknown sample
g.The use of molecular genetics in identifying of viral pathogens
h.Sequencing DNA; public databases within molecular biology studies

4.Genetic manipulations to improve the performance of plants (allowance 2/0)
5.Tissue culture and their use (allowance 10/16)
a.Elimination of viral pathogens using in vitro, legislation
b.Isolation of meristem , meristem culture , micrografting
c.The use of in vitro techniques in plant breeding ( in vitro selection , somaclonal variation , genetic manipulation)
d.Micropropagation of horticultural plant species

6.Excursion in the privat biotechnological company (allowance 0/4)

Learning outcomes and competences:
Generic competences:
-ability to apply knowledge
-ability to create new ideas (creativity)
-ability to work independently
-science and research skills
-work in team

Specific competences:
-Application skills of professional knowledge
-Higher professional knowledge
-Stuents will learn the principles of operation of instruments and techniques within the field of plant biotechnology

Type of course unit:optional
Year of study:Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement:There is no compulsory work placement in the course unit.
Recommended study modules:none
Assessment methods:credit requirements: prerequisite for credit is participation in the exercises to the extent of at least 82% of the teaching plan + written test with a score of at least 66% of the maximum available points.

final exam: verification of acquired knowledge in the form of an oral examination; qualified response to at least of 70% of asked questions is required.
Learning activities and study load (hours of study load)
Type of teaching methodDaily attendance
Direct teaching
     lecture28 h
     practice28 h
     laboratory work56 h
     consultation6 h
     public presentation (oral)2 h
     preparation for exam26 h
     preparation of presentation12 h
     elaboration of reports10 h
Total168 h

Basic reading list
  • BARÁNEK, M. -- MORAVCOVÁ, K. -- PIDRA, M. Biotechnologie v zahradnictví - návody pro praktická cvičení. ediční středisko MZLU v Brně: Mendelova zemědělská a lesnická univerzita v Brně, 2006. 47 p. ISBN 80-7157-937-8.
  • ROSYPAL, S. Úvod do molekulární biologie. Brno: Stanislav Rosypal, Brno, 2002. 1199 p. ISBN 80-902562-1-X.
  • ROSYPAL, S. Úvod do molekulární biologie. ISBN 80-902562-2-8.
  • GRAY, D J. Plant development and biotechnology. Boca Raton: CRC Press, 2005. 358 p. ISBN 0-8493-1614-6.
  • TRIGIANO, R. Plant Tissue Culture Concepts and Laboratory Exercises. USA, Florida: CRS press, 1996. 564 p. ISBN 0-8493-9409-0.