Study programmes / C-HE Horticultural Engineering / Practics From Biotechnology
Course code:PZB
Course title in language of instruction:Praktika z biotechnologií
Course title in Czech:Praktika z biotechnologií
Course title in English:Practics From Biotechnology
Mode of completion and number of credits:Fulfillment of requirements (2 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes:full-time, 0/2
(full-time, hours of lectures per week / hours of seminars per week)
Language of instruction:Czech
Level of course:master; master continuing
Semester:SS 2013/2014 - FH
Name of lecturer:doc. Mgr. Miroslav Baránek, Ph.D. (instructor, lecturer, supervisor)
Ing. Jana Čechová, Ph.D. (instructor)
prof. RNDr. Miroslav Pidra, CSc. (lecturer)
Mgr. Jana Raddová, Ph.D. (instructor, lecturer)
Aims of the course:To acquaint students with recently used methods in biotechnology. Show to students basic equipment of biotechnological laboratory and learn them to work with its usual equipment. Obtained knowledges open view about possible practical applications of this branch of methods in horticulture.
Course contents:
1.Usual equipment of laboratory for molecular genetics - introduction (allowance 0/2)
2.Isoaltion of nucleic acids from plant tissues (allowance 0/2)
3.Polymerase chain reaction (PCR) - theory and its preparation (allowance 0/2)
4.Basic molecular genetics methods working on the base of fragmentation analysis - RFLP, RAPD, SSR, AFLP and its derived methods (allowance 0/2)
5.Utilisation of genetic markers for studies of genetic similarity (allowance 0/4)
6.Utilisation of SSR markers for identification of variety (allowance 0/4)
7.Utilisation of Real Time PCR for quantification of GMO material in unknown sample (allowance 0/2)
8.Utilisation of molecular genetics for identification of plant viruses in plant material (allowance 0/4)
9.DNA sequencing, public databases of sequences (allowance 0/2)

Learning outcomes and competences:
Generic competences:
-ability to analyse and synthesize
-ability to apply knowledge
-ability to communicate with professionals in different field of study
-ability to create new ideas (creativity)
-ability to solve problems
-ability to speak and write in mother tongue
-ability to work in international context
-ability to work independently
-capacity to adapt to new situations
-capacity to learn
-communication in second language
-ethical commitment
-general knowledge
-professional knowledge
-quality concept awareness
-science and research skills
-skilled at utilizing and processing information
-work in team

Specific competences:
-ability to use theoretical knowledge of the field and gain practical knowledge and laboratory skills
-Knowledge of the principles of safety work in molecular laboratory
-The ability to interpret the results of molecular analysis
-the ability to propose and realize the simple molecular-genetic analyses

Type of course unit:optional
Year of study:Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement:There is no compulsory work placement in the course unit.
Recommended study modules:none
Assessment methods:credit: the requierement to obtain credit is to achieve at least 66% of points in the written test entered at the end of teaching period
Learning activities and study load (hours of study load)
Type of teaching methodDaily attendance
Direct teaching
     practice14 h
     laboratory work14 h
     preparation for regular assessment14 h
     preparation for regular testing14 h
Total56 h

Basic reading list
  • Plant Molecular Biology - A Laboratory Manual. Berlin: Springer, 1997. 29 p. Springer Lab Manual. ISBN 3-540-58405-6.
  • BARÁNEK, M. -- MORAVCOVÁ, K. -- PIDRA, M. Biotechnologie v zahradnictví - návody pro praktická cvičení. ediční středisko MZLU v Brně: Mendelova zemědělská a lesnická univerzita v Brně, 2006. 47 p. ISBN 80-7157-937-8.
  • CHOPRA, V /. Applied Plant Biotechnology. Enfield: Science Publishers, 1999. 5 p. ISBN 1-57808-033-9.