Study programmes / C-HE Horticultural Engineering / Breeding of Plants and Biotechnology
Course code:SRB
Course title in language of instruction:Šlechtění rostlin a biotechnologie
Course title in Czech:Šlechtění rostlin a biotechnologie
Course title in English:Breeding of Plants and Biotechnology
Mode of completion and number of credits:Exam (4 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes:full-time, 2/0
(full-time, hours of lectures per week / hours of seminars per week)
Language of instruction:Czech
Level of course:bachelor; master continuing
Semester:WS 2018/2019 - FH
Name of lecturer:doc. Mgr. Miroslav Baránek, Ph.D. (examiner, lecturer, supervisor)
Ing. Kateřina Baránková, Ph.D. (examiner, lecturer)
Ing. Jakub Pečenka (lecturer)
Ing. Bc. Eliška Peňázová (lecturer)
Mgr. Jana Raddová, Ph.D. (lecturer)
Ing. Miroslav Vachůn, Ph.D. (lecturer)
Aims of the course:Based on our knowledge of genetics and molecular genetics to acquaint students with traditional and modern breeding methods and procedures point to the potential use of genetic varieties praxi.Second part of the course focuses on using the latest molecular techniques and procedures in practical plant breeding .
Course contents:
1.Introduction and basic concepts of classical plant breeding. (allowance 4/0)
a.The status of plant breeding in agriculture, the importance of breeding, history and development of plant breeding in the world and us. Genetic structure of the variety, variability of cultivated plants, important cultural properties and characteristics of flora
b.The main phases of the breeding process, genetic resources, centers of origin of cultivated plants, preservation and conservation of genetic resources. Implications for the propagation of breeding methods and selection procedures, techniques and procedures for crossing

2.Conventional techniques, methods and practices of breeding. (allowance 6/0)
a.The techniques and selection methods. Breeding methods for self, for cross-pollinated, in vegetatively propagated. Peculiarities of the biennial and perennial species.
b.Nature of heterosis, heterosis theory explaining, using heterózního effect in plant-breeding techniques for the breeding of F1 hybrids. Male sterility, genetic determination of male sterility, the use of male sterility in breeding F1 hybrids

3.Alternative breeding techniques (allowance 6/0)
a.Mutation breeding, induced mutagenesis, mutagens used, methods of working. Remote hybridization causes problems with pollination of species and the possibility of overcoming, the properties of distant hybrids
b.Properties of polyploids, the use of polyploidy in plant breeding, methods of obtaining polyploid breeding, use of aneuploidy. haploid and in plant breeding. Breeding for resistance to pests and diseases, genetic nature of resistance

4.Molecular markers, their nature and use (allowance 6/0)
a.Hybridization techniques used to detect molecular markers. RFLP, VNTR, FISH and more
b.Techniques based on polymerase chain reaction (PCR): RAPD, SSR, AFLP
c.Methods for DNA sequencing
d.The use of molecular markers to analyze genetic resources (genotyping) and using selection markers (marker assisted selection, MAS)

5.Gene manipulation in plant breeding (allowance 6/0)
a.The basic strategy of gene manipulation in plants, gene cloning and cloning vectors, expression vectors
b.The use of Agrobacterium tumefaciens in Transgenosis plants, other technologies can obtain genetically modified plants.
c.Objectives Transgenosis in plants, most frequently used genes, characteristics of the GMOs, placing GMOs on the market.
d.European and Czech legislation governing the handling of GMOs

Learning outcomes and competences:
Generic competences:
-ability to analyse and synthesize
-ability to apply knowledge
-ability to communicate with professionals in different field of study
-ability to create new ideas (creativity)
-ability to solve problems
-ability to speak and write in mother tongue
-ability to work in interdisciplinary team
-ability to work in international context
-ability to work independently
-capacity to adapt to new situations
-capacity to learn
-communication in second language
-ethical commitment
-general knowledge
-professional knowledge
-quality concept awareness
-science and research skills
-skilled at utilizing and processing information

Specific competences:
-Ability to compile a methodology for the experiment, implement the experiment and evaluate it
-The ability to draw conclusions applicable in breeding from the results of molecular analysis
-The ability to propose an approach for the desired properties of the plants with the classical and biotechnological methods.
-The ability to propose how to reach the projected plant traits by means of classical and biotechnological methods.
-The ability to select a suitable molecular marker for the plant species for the MAS (Marker assisted Sellection)

Type of course unit:required
Year of study:Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement:There is no compulsory work placement in the course unit.
Recommended study modules:none
Assessment methods:credit: written test (at least 66 % of right anwers)

Examination: written test (must achieve 80% correct answers), oral exam
Learning activities and study load (hours of study load)
Type of teaching methodDaily attendance
Direct teaching
     lecture28 h
     consultation4 h
     preparation for exam55 h
     preparation for regular assessment25 h
Total112 h

Basic reading list
  • Šlechtění rostlin: (obecná část). 1st ed. České Budějovice: JU ZF, 1997. 133 p. ISBN 80-7040-255-5.
  • CHLOUPEK, O. Genetická diverzita, šlechtění a semenářství. 3rd ed. Praha: Academia, 2008. 307 p. ISBN 978-80-200-1566-2.
  • BARÁNEK, M. -- MORAVCOVÁ, K. -- PIDRA, M. Biotechnologie v zahradnictví - návody pro praktická cvičení. ediční středisko MZLU v Brně: Mendelova zemědělská a lesnická univerzita v Brně, 2006. 47 p. ISBN 80-7157-937-8.
Recommended reading list
  • EHRENBERGEROVÁ, J. Odrůdy, osivo a sadba. 1st ed. Brno: Mendelova univerzita v Brně, 2014. 105 p. ISBN 978-80-7509-003-4.
  • SMUTNÁ, P. Šlechtění rostlin a semenářství: návody do cvičení. 1st ed. Brno: Mendelova univerzita v Brně, 2014. 127 p.