Study programmes / B-PPR Plant Protection / Biochemistry - CV
Course code:CBI-C
Course title in language of instruction:Biochemie - CV
Course title in Czech:Biochemie - CV
Course title in English:Biochemistry - CV
Mode of completion and number of credits:Fulfillment of requirements (2 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes:full-time, 0/2
(full-time, hours of lectures per week / hours of seminars per week)
Language of instruction:Czech
Level of course:bachelor
Semester:SS 2018/2019 - FA
Name of lecturer:prof. RNDr. Vojtěch Adam, Ph.D. (instructor)
Ing. Žaneta Buchtová (examiner, instructor)
Mgr. Natalia Cernei, Ph.D. (examiner, instructor)
Mgr. Roman Guráň, Ph.D. (examiner, instructor)
Ing. Jiří Kudr, Ph.D. (examiner, instructor)
Ing. Zuzana Lacková (examiner, instructor)
doc. RNDr. Ondřej Zítka, Ph.D. (examiner, instructor, supervisor)
Prerequisites:none
 
Aims of the course:Getting the basic information and skills in the biochemical laboratory work.
Course contents:
1.Laboratory exercise 1 (allowance 0/4)
 
a.Determination of vitamin C in biological sample
b.Calibration curves
c.Chromatography of carotenoic dyes

2.Laboratory exercise 2 (allowance 0/4)
 
a.DNA isolation
b.RNA isolation
c.Analyse of green dyes

3.Laboratory exercise 3 (allowance 0/4)
 
a.Protein dialysis
b.Protein isolation
c.Electrophoretic determination of portein from blood serum

4.Laboratory exercise 4 (allowance 0/4)
 
a.Direct and gradual extraction of proteins
b.Calibration curves

5.Laboratory exercise 5 (allowance 0/4)
 
a.Enzyme kinetic
b.Calibration curves

6.Laboratory exercise 6 (allowance 0/4)
 
a.Determination of aminotransferase activity in a biological sample
b.Assessment of amylase activity

Learning outcomes and competences:
Generic competences:
 
-ability to work independently
-skilled at utilizing and processing information

Specific competences:
 
-Ability of performing of basic chemical laboratory methods
-Ability to determine enzyme activity in the sample
-Ability to understand and work with basic statistical quantities describing the experimental results
-Competences for practical isolation of biological macromolecules (DNA, RNA, proteins, ...)
-Knowledge of the methods of chromatography and electrophoresis

Type of course unit:required
Year of study:Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement:There is no compulsory work placement in the course unit.
Recommended study modules:none
Assessment methods:For the award of credit is required to participate in all laboratory exercises and successful completion of three control tests during the semester (each must meet the 60%, 10 questions, 40 min.).
 
Learning activities and study load (hours of study load)
Type of teaching methodDaily attendance
Direct teaching
     practice28 h
Self-study
     preparation for regular testing14 h
     elaboration of reports14 h
Total56 h

Basic reading list
  • ZEHNÁLEK, J. Biochemie: cvičení. 2nd ed. Brno: Mendelova zemědělská a lesnická univerzita v Brně, 2005. 72 p. ISBN 80-7157-867-3.
  • KODÍČEK, M. -- VALENTOVÁ, O. Laboratorní cvičení z biochemie. Nakladatelství Olomouc, s.r.o., 2000. 203 p. ISBN 80-7182-104-7.
Recommended reading list
  • ZEHNÁLEK, J. Biochemie 2. Brno: Mendelova zemědělská a lesnická univerzita v Brně, 2007. 200 p. ISBN 978-80-7157-716-4.
  • ZEHNÁLEK, J. Chemie a biochemie. 1st ed. Brno: VŠZ, 1994. 165 p. ISBN 80-7157-135-0.