Study programmes / B-PPR Plant Protection / Diagnostic methods
Course code:DIME
Course title in language of instruction:Diagnostické metody
Course title in Czech:Diagnostické metody
Course title in English:Diagnostic methods
Mode of completion and number of credits:Exam (5 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes:full-time, 2/2
(full-time, hours of lectures per week / hours of seminars per week)
Language of instruction:Czech
Level of course:bachelor
Semester:SS 2020/2021 - FA
Name of lecturer:Mgr. Ing. Eva Hrudová, Ph.D. (instructor, lecturer)
prof. Ing. Radovan Pokorný, Ph.D. (examiner, instructor, lecturer, supervisor)
Ing. Jana Víchová, Ph.D. (instructor)
Aims of the course:Student obtain knowledge in theoretical bases of diagnostics methods of pathogens and pests and skill in performing of these methods. Lectures will be aimed at macroscopical, microscopical, cultivation, serological and molecular methods
Course contents:
1.Diagnostics of virus pathogens (allowance 2/2)
a.Symptomatology, host range, test plants
b.Serology - basis of immunology, praparation of antibodies, methods
c.Nucleic acids determination - PCR, molecular hybridisation
d.Electron microscopy

2.Diagnostics of bacterial pathogens (allowance 2/2)
a.Biological, microscopical, and cultivation methods
b.Physiological, biochemical, and chemical methods
c.Serological and molecular methods

3.Diagnostics of fungi pathogens (allowance 4/4)
a.Biological, microscopical, and cultivation methods. Symptomatology
b.Serological and molecular methods
c.Diagnostics of fungi secondary metabolits

4.Diagnostics of damage of pests (allowance 4/4)
a.Determination of plant injuries
b.Determination of pests
c.Insects preparation methods

5.Diacnostics protocols of pathogens (allowance 2/2)
6.Certification schemes (allowance 2/2)
7.Excursions to relevant diagnostics laboratories (allowance 12/12)

Learning outcomes and competences:
Generic competences:
-ability to apply knowledge
-ability to solve problems
-ability to work in interdisciplinary team
-ability to work independently
-skilled at utilizing and processing information

Specific competences:
-Ability of performing pathogen diagnostics
-Ability of performing pest diagnostics
-Knowledge of diagnostics protocols of pathogens and pests
-Knowledge of plant damage by insects, mites and nematodes
-Skills of utilization and application of basic molecular and serological methods

Type of course unit:required
Year of study:Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement:There is no compulsory work placement in the course unit.
Recommended study modules:none
Assessment methods:participation on seminars (80%), submission of a two protocols during the semester (20% of total mark), oral exam 30 minutes (80% of total mark)
Learning activities and study load (hours of study load)
Type of teaching methodDaily attendance
Direct teaching
     lecture28 h
     practice16 h
     professional educational excursion12 h
     preparation for exam40 h
     elaboration of reports44 h
Total140 h

Basic reading list
  • KAZDA, J. et al. Choroby a škůdci polních plodin, ovoce a zeleniny. 3rd ed. Praha: Martin Sedláček, 2003. 158 p. ISBN 80-86726-03-7.
  • KŮDELA, V. -- KOCOUREK, F. et al. Seznam škodlivých organismů rostlin: viry, prokaryota, houby a houbám podobné organismy, živočišní škůdci, plevele a parazitické rostliny :. 1st ed. Praha: Agrospoj, 342 p. ISBN 80-7084-232-6.
  • ŠMARDA, J. et al. Metody molekulární biologie. 1st ed. Brno: Masarykova univerzita, 2008. 188 p. ISBN 978-80-210-3841-7.
Recommended reading list
  • FERENČÍK, M. et al. Biochemické laboratórne metódy. 1st ed. Bratislava: Alfa, 1981. 852 p. Edícia potravinárskej literatúry.
  • Imunochémia. 2nd ed. Bratislava: Alfa, 1989. 591 p. ISBN 80-05-00043-X.
  • TRIGIANO, R N. Plant pathology: concepts and laboratory exercises. Boca Raton, Fla.: CRC Press, 413 p. ISBN 0-8493-1037-7.