Study programmes / C-FT Chemistry and Food Technology / Bioanalytical Methods
Course code:CBAN
Course title in language of instruction:Bioanalytické metody
Course title in Czech:Bioanalytické metody
Course title in English:Bioanalytical Methods
Mode of completion and number of credits:Exam (5 credits)
(1 ECTS credit = 28 hours of workload)
Mode of delivery/Timetabled classes:full-time, 2/2
(full-time, hours of lectures per week / hours of seminars per week)
Language of instruction:Czech
Level of course:master continuing
Semester:WS 2019/2020 - FA
Name of lecturer:prof. RNDr. Vojtěch Adam, Ph.D. (instructor, lecturer, supervisor)
Ing. Soňa Křížková, Ph.D. (instructor, lecturer)
Ing. Lukáš Nejdl, Ph.D. (instructor, lecturer)
Ing. Eliška Sedláčková (instructor, lecturer)
Ing. Kristýna Šmerková, Ph.D. (instructor, lecturer)
doc. Mgr. Markéta Vaculovičová, Ph.D. (examiner, instructor, lecturer)
Ing. Tereza Vaněčková (instructor, lecturer)
Prerequisites:none
 
Aims of the course:Students should learn about principles of modern bioanalytical methods and their practical application.
Course contents:
1.Enzymatic analytical methods (allowance 4/4)
 
a.Kinetics of enzymatic reactions
b.Units of enzymatic activity
c.Stabilisation of enzymes
d.Enzymes as analytical agents

2.Immunochemical methods (allowance 4/4)
 
a.Antigens, antibodies and their preparation
b.Conjugates in immunochemistry
c.Interactions of antibodies with antigens
d.Precipitation and non-precipitation methods
e.Application in analytics

3.Affinity chromatography (allowance 3/3)
 
a.Bioaffinity chromatography
b.Non-specific affinity chromatography
c.Other extraction techniques based on affinity relationships

4.Electromigration (electrophoretic) methods (allowance 4/4)
 
a.Principle of electromigration methods
b.Free-flow, zonal, equlibrium and capillary electrophoresis
c.Analytical applications of electrophoretic methods

5.Methods of nucleic acids extraction (allowance 2/4)
 
a.Differences in DNA and RNA extraction methods
b.Phenol-chlorophorm extraction
c.Use of extraction kits

6.In vitro DNA amplification by polymerase chain reaction (PCR) (allowance 4/5)
 
a.DNA - template for PCR
b.In vitro DNA synthesis
c.Detection of PCR products
d.Identification of genetically modified organisms by means of PCR

7.Electrochemical methods (allowance 4/4)
 
a.Methods of studies on low-molecular substances -- analysis of heavy metals and important secondary metabolites
b.Methods of studies on low-molecular substances -- analysis of nucleic acids and proteins

8.Biochips and biosensors (allowance 2/0)
9.Nanotechnologies and nanomaterials (allowance 1/0)

Learning outcomes and competences:
Generic competences:
 
-ability to analyse and synthesize
-ability to apply knowledge
-ability to work independently
-professional knowledge
-science and research skills

Specific competences:
 
-Ability to choose and use a bioanalytical method for particular practical applications
-Basic application of modern bioanalytical methods in nanotechnology
-Knowledge of basic concepts in the field of bioanalytical methods
-Knowledge of nucleic acids isolation and amplification methods
-The ability to use enzymatic, immunochemical and electromigration methods in the practical analysis of real sample

Type of course unit:required
Year of study:Not applicable - the subject could be chosen at anytime during the course of the programme.
Work placement:There is no compulsory work placement in the course unit.
Recommended study modules:none
Assessment methods:Examination. The pre-requisition of an exam is an acquisition of a laboratory practicals credit.
Practical labs requirements:

100% presence, only medical reasons supported by the medical report can be
accepted. In case of absence without any medical report, 5-page assay on the
topic of the presented method must be provided. I tis necessary to provide
all protocols (1 per group, checked and accepted by the tutor). At the end
of the semester - test has to be completed (60% to pass)

Exam requirements:

Written test - 30 questions, 70% correct to pass, in case of reaching 70%
but interest in better mark, mark can be improved by oral examination
 
Learning activities and study load (hours of study load)
Type of teaching methodDaily attendance
Direct teaching
     lecture28 h
     practice28 h
     consultation12 h
Self-study
     preparation for exam50 h
     preparation for regular testing10 h
     elaboration of reports12 h
Total140 h

Basic reading list
  • RAUCH, P. -- KRÁLOVÁ, B. -- FUKAL, L. Bioanalytická chemie. Praha: VŠCHT, 2007. 254 p. ISBN 80-7080-449-1.
  • KLOUDA, P. Moderní analytické metody. 2nd ed. Ostrava: Pavel Klouda, 2003. 132 p. ISBN 80-86369-07-2.
Recommended reading list
  • KODÍČEK, M. -- KARPENKO, V. Biofysikální chemie. 2nd ed. Praha: Academia, 2000. 337 p. ISBN 80-200-0791-1.
  • KODÍČEK, M. -- VALENTOVÁ, O. Laboratorní cvičení z biochemie. Nakladatelství Olomouc, s.r.o., 2000. 203 p. ISBN 80-7182-104-7.
  • MARKUŠOVÁ, K. Elektrochemické metódy. Košice: Univerzita Pavla Jozefa Šafárika v Košiciach, 2000. 150 p.
  • SKLÁDAL, P. Biosensory. Brno: Masarykova univerzita, 1999. 149 p.